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3,3’,5,5’-Tetramethylbenzidine

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3,3’,5,5’-Tetramethylbenzidine

Systematic name 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine
Chemical formula C16H20N2
Molecular mass 240.3482 g/mol
Density x.xxx g/cm3
Melting point 192–196 °C
Boiling point xx.x °C
CAS number [54827-17-7]
SMILES NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C
[Chemical infoboxDisclaimer and references]

3,3’,5,5’-Tetramethylbenzidine or TMB is a reagent used in staining procedures in immunohistochemistry.

It is a white crystal powder which should be protected from exposure to sunlight, and a mutagen.

Forms a blue mixture when combined with a peroxidase substrate; completion of the reaction results in a yellow coloration.

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