Anna was a spinster when her brother King Zygmunt II August died. In 1576 she married Stefan Batory (1533-1586), then Reigning Prince of Transylvania, who that same year was elected King of Poland and Grand Duke of Lithuania. Anna survived her husband and died childless.
Her heir was the only son of her youngest sister, Catherine of Poland, Queen of Sweden: Sigismund. Anna helped him, after her husband's death, gain the Polish throne as Sigismund III Vasa.
Anna died as Dowager Queen of Poland during her nephew Zygmunt's reign, in her own country where she had been born and had lived.
Anna Jagiellon is one of the persons who figure in a famous painting by Jan Matejko depicting the preaching of Piotr Skarga.