Cell fractionation

Encyclopedia : C : CE : CEL : Cell fractionation

Cell fractionation is the separation of homogeneous sets - usually organelles - from a heterogeneous population of cells.

Contents

1 Steps

1.1 Homogenisation
1.2 Filtration
1.3 Purification

2 See also
3 External links

Steps

Disruption (homogenisation) of cells and liberation of organelles.
Macro Filtration
Purification of cell components.

Homogenisation

Tissue is typically homogenised in an isotonic buffer solution using a variety of mechanisms. A 'Potter-Elvehjem homogeniser' is often used as it is relatively gentle. Other procedures include grinding, mincing, chopping, pressure changes, osmotic shock and freeze-thawing.

Consult Cell disruption for further details.

Filtration

This step may not be necessary depending on the source of the cells. Animal tissue however is likely to yield connective tissue which must be removed. Commonly, filtration is achieved either by pouring through gauze or with a suction filter and the relevant grade ceramic filter.

Purification

Invariably achieved by Differential centrifugation - the sequential increase in gravitational force resulting in the sequential separation of organelles according to their density.

External links

[Microfluidicscorp - MFIC OTC]