Gli2

Encyclopedia : G : GL : GLI : Gli2

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Gli2 is a transcriptional activator and repressor of which there are four isophorms; Gli2 alpha, beta, gamma and delta (Tojo et al., 2003a). C-terminal transcriptional activator and N-terminal repressor regions have been identified in both Gli2 and Gli3 ((Sasaki et al., 1999) However, N-terminal part of human GLI2 is much smaller than its mouse or frog homologs, suggesting that it may lack repressor function. Gli2 affects ventroposterior mesodermal development by regulating at least three different genes; Wnt genes involved in morphogenesis, Brachyruy genes involved in tissue specification and Xhox3 genes involved in positional information ((Altaba et al., 2003) II.23). The anti apoptotic protein BCL-2 is up regulated by Gli2 and to a lesser extent Gli1 but not Gli3 which may lead to carcinogenesis (Regl et al., 2004). It has been shown in mouse models that Gli1 can compensate for knocked out Gli2 function when expressed from the Gli2 locus. This suggests that in mouse embryogenesis, Gli1 and Gli2 regulate a similar set of target gene. Mutations do develop later in development suggesting Gli1/Gli2 transctiptional regulation is context dependent (Regl et al., 2004) Gli2 and Gli3 are important in the formation and development of lung, trachea and oesophagus during embryo development (Motoyama et al., 1998) Studies have also shown that GLI2 plays a dual role as activator of keratinocyte proliferation and repressor of epidermal differentiation (Regl et al., 2004a)

There is a significant level of cross talk and functional overlap between the Gli TF’s. Gli2 has been shown to compensate for the loss of Gli1 in transgenic Gli1-/- mice which are phonotypically normal (Ding et al., 1998). However, loss of Gli3 leads to abnormal patterning and loss of Gli2 affects the development of ventral cell types, most significantly in the floor plate. Gli2 has been shown to compensate for Gli1 ventrally and Gli3 dorsally in transgenic mice (Litingtung and Chiang, 2000). Gli2 null mice embryos develop neural-tube defects which, can be rescued by overexpression of Gli1 (Jacob and Briscoe, 2003). Interestingly Gli1 has been shown to induce the two GLI2 α/β isoforms.

Transgenic double homozygous Gli1-/- and Gli2-/- knockout mice display serious central nervous system and lung defects have small lungs, undescended testes, and a hopping gait as well as an extra postaxial nubbin on the limbs (Park et al., 2000). Gli2-/- and Gli3-/- double homozygous transgenic mice are not viable and do nos survive bond embryonic level (Mo et al., 1997); (Hardcastle et al., 1998); (Motoyama et al., 1998). These studies suggest overlapping roles for Gli1 with Gli2 and Gli2 with Gli3 in embryonic development.

Interestingly, transgenic Gli1-/- and Gli2-/- mice have a similar phenotype to transgenic Gli1 gain of function mice. This phenotype includes failure to thrive, early death, and a distended gut although no tumors form in transgenic Gli1-/- and Gli2-/- mice. This could suggest that overexpression of human Gli1 in the mouse may have led to a dominant negative rather than a gain-of-function phenotype (Yang et al., 1997); (Park et al., 2000)

Transgenic mice over expressing the transcription factor Gli2 under the K5 promoter in cutaneous keratinocytes develop multiple BCC like skin tumours on the ears tail trunk and dawsal aspect of the paw. Unlike Gli1 transgenic mice, Gli2 transgenic mice only developed BCC like tumors. Transgenic mice with N-terminal deletion of Gli2, developed the benign trichoblastomas, cylindromas and hamartomas but rarely developed BCCs (Sheng et al., 2002) Gli2 is expressed in the interfollicular epidermis and the outer root sheath of hair follicles in humannormal skin. This is significant as Shh regulates hair follicle growth and morphogenesis. When inappropriately activated causes hair follicle derived tumors, the most clinically significant being the BCC (Oro and Higgins, 2003).

Of the four Gli2 isoforms the expression of Gli2beta mRNA was increased the most in BCCs. Gli2beta is an isoform spliced at the first splicing site which contains a repression domain and consists of an intact activation domain. Overexpression of this Gli2 splice variant may lead to the upregulation of the Shh signalling pathway, thereby inducing BCCs. (Tojo et al., 2003b)

In human keratinocytes Gli2 activation upregulates a number of genes involved in cell cycle progression including E2F1, CCND1, CDC2 and CDC45L. Gli2 is able to induce G1–S phase progression in contact-inhibited keratinocytes which may drive tumour development. (Regl et al., 2004b)

Although both Gli1 and Gl12 have been implicated it is unclear whether one or both are needed for carcinogenesis. However, due to feed back loops, one may directly or indirectly induce the other.

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